1DCK
STRUCTURE OF UNPHOSPHORYLATED FIXJ-N COMPLEXED WITH MN2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-01-01 |
Detector | MARRESEARCH |
Spacegroup name | P 1 |
Unit cell lengths | 31.700, 42.500, 44.700 |
Unit cell angles | 93.20, 103.00, 101.80 |
Refinement procedure
Resolution | 14.450 - 2.000 |
R-factor | 0.206 |
Rwork | 0.206 |
R-free | 0.25300 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.051 | 0.196 |
Total number of observations | 24325 * | |
Number of reflections | 13062 | |
<I/σ(I)> | 15.6 | |
Completeness [%] | 87.0 | 57.7 |
Redundancy | 1.9 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | PEG1500, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 4K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 12 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 20 (mM) | |
3 | 1 | drop | EDTA | 0.1 (mM) | |
4 | 1 | drop | dithiothreitol | 1 (mM) | |
5 | 1 | reservoir | PEG1500 | 40 (%(w/v)) | |
6 | 1 | reservoir | Tris-HCl | 20 (mM) |