1DCA

STRUCTURE OF AN ENGINEERED METAL BINDING SITE IN HUMAN CARBONIC ANHYDRASE II REVEALS THE ARCHITECTURE OF A REGULATORY CYSTEINE SWITCH

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	42.700, 41.700, 73.000
Unit cell angles	90.00, 104.60, 90.00

Refinement procedure

Resolution	7.000 * - 2.200
R-factor	0.182
RMSD bond length	0.014
RMSD bond angle	1.100
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.200 *
Rmerge	0.056 *
Total number of observations	14756 *
Number of reflections	10345 *
Completeness [%]	72.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	0.3 (mM)
2	1	drop	Tris-HCl	50 (mM)
3	1	drop		150 (mM)
4	1	drop		3 (mM)
5	1	reservoir	Tris-HCl	50 (mM)
6	1	reservoir		150 (mM)
7	1	reservoir		3 (mM)
8	1	reservoir	ammonium sulfate	1.75-2.5 (M)