1DC6
STRUCTURAL ANALYSIS OF GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE FROM ESCHERICHIA COLI: DIRECT EVIDENCE FOR SUBSTRATE BINDING AND COFACTOR-INDUCED CONFORMATIONAL CHANGES.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-07-18 |
| Detector | MAC Science DIP-2030 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 85.450, 134.470, 67.340 |
| Unit cell angles | 90.00, 107.89, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.224 * |
| Rwork | 0.224 |
| R-free | 0.29100 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.421 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.050 |
| High resolution limit [Å] | 1.900 | 2.010 |
| Rmerge | 0.060 | 0.317 |
| Total number of observations | 660366 * | |
| Number of reflections | 51859 | |
| <I/σ(I)> | 19.6 | |
| Completeness [%] | 91.4 | 48.1 * |
| Redundancy | 12.73 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 * | 18 * | PEG 4000, magnesium chloride, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 20 (mM) | |
| 2 | 1 | drop | 200 (mM) | ||
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | drop | protain | 20 (mg/ml) | |
| 6 | 1 | reservoir | PEG4000 | 15 (%) | |
| 7 | 1 | reservoir | 0.8-0.9 (M) | ||
| 8 | 1 | reservoir | Tris-HCl | 0.1 (M) |
