1DC3
STRUCTURAL ANALYSIS OF GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE FROM ESCHERICHIA COLI: DIRECT EVIDENCE FOR SUBSTRATE BINDING AND COFACTOR-INDUCED CONFORMATIONAL CHANGES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-04-30 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | I 41 |
| Unit cell lengths | 119.570, 119.570, 158.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.24100 |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.370 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.540 |
| High resolution limit [Å] | 2.400 | 2.500 |
| Rmerge | 0.036 * | 0.216 |
| Total number of observations | 1055831 * | |
| Number of reflections | 38265 | |
| <I/σ(I)> | 14.5 | |
| Completeness [%] | 99.8 * | 100 |
| Redundancy | 27.6 | 5.36 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 * | 18 * | PEG 4000, MAGNESIUM CHLORIDE, TRIS-HCL, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 20 (mM) | |
| 2 | 1 | drop | 200 (mM) | ||
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | drop | protain | 20 (mg/ml) | |
| 6 | 1 | reservoir | PEG4000 | 15 (%) | |
| 7 | 1 | reservoir | 0.8-0.9 (M) | ||
| 8 | 1 | reservoir | Tris-HCl | 0.1 (M) |
