1DBZ
C153S MUTANT OF PEA FRUCTOSE-1,6-BISPHOSPHATASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE D41A |
Synchrotron site | LURE |
Beamline | D41A |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 1999-01-01 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 78.900, 114.500, 94.500 |
Unit cell angles | 90.00, 114.50, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.650 |
Rwork | 0.235 |
R-free | 0.29100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | OXIDIZED PEA WILD TYPE FBPASE FORM 1 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.360 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.000 | 2.700 |
High resolution limit [Å] | 2.600 | 2.650 |
Rmerge | 0.096 | 0.900 |
Total number of observations | 291800 * | |
Number of reflections | 41334 | |
<I/σ(I)> | 13 | |
Completeness [%] | 87.6 | 89 |
Redundancy | 7 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 20% PEG 1000, 50MM NA ACETATE (PH 5), 50 MM MGCL2, 5MM F6P, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG1000 | 18-20 (%) | |
2 | 1 | reservoir | sodium acetate | 50 (mM) | |
3 | 1 | reservoir | 50 (mM) | ||
4 | 1 | reservoir | F6P | 5 (mM) |