1DBV
GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE MUTANT WITH ASP 32 REPLACED BY GLY, LEU 187 REPLACED BY ALA, AND PRO 188 REPLACED BY SER COMPLEXED WITH NAD+
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 297 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-04 |
| Detector | MAC Science DIP-2020 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.950, 124.680, 82.870 |
| Unit cell angles | 90.00, 108.63, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.14 |
| Rwork | 0.140 |
| R-free | 0.21400 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 25.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 15.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.068 * |
| Number of reflections | 45468 |
| Completeness [%] | 81.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.9 | pH 6.9 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 2 (M) | |
| 3 | 1 | drop | NAD(P)+ | 30 (mM) | |
| 4 | 1 | drop | phosphate | 50 (mM) | |
| 5 | 1 | drop | EDTA | 1 (mM) | |
| 6 | 1 | drop | dithiothreitol | 1 (mM) | |
| 7 | 1 | reservoir | ammonium sulfate | 2.5 (M) |
