1DBU

Crystal structure of cysteinyl-tRNA(Pro) deacylase protein from H. influenzae (HI1434)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	1998-10-27
Detector	BRANDEIS
Spacegroup name	C 1 2 1
Unit cell lengths	102.570, 42.490, 32.540
Unit cell angles	90.00, 104.17, 90.00

Refinement procedure

Resolution	20.000 - 1.800
Rwork	0.193
R-free	0.26200
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	0.032
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.059	0.357
Total number of observations	47185 *
Number of reflections	25089
Completeness [%]	98.7	98

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *		drop consists of equal volume of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	drop	potassium phosphate	50 (mM)
3	1	drop	EDTA	0.1 (mM)
4	1	drop	dithiothreitol	5 (mM)
5	1	reservoir	Tris-HCl	100 (mM)
6	1	reservoir	mPEG5000	25-35 (%)
7	1	reservoir	propanol	3 (%)