1DBS

MECHANISTIC IMPLICATIONS AND FAMILY RELATIONSHIPS FROM THE STRUCTURE OF DETHIOBIOTIN SYNTHETASE

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	72.874, 49.000, 61.309
Unit cell angles	90.00, 106.38, 90.00

Refinement procedure

Resolution	7.000 * - 1.800
R-factor	0.179
Rwork	0.179
RMSD bond length	0.012
RMSD bond angle	23.150 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	7.000
High resolution limit [Å]	1.800
Rmerge	0.034 *
Number of reflections	18656
Completeness [%]	98.0	92.3 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	10 (mg/ml)
2	1	drop	cacodylate-HCl	50 (mM)
3	1	reservoir	cacodylate-HCl	50 (mM)
4	1	reservoir	ammonium sulfate	0.5 (M)