1DBH

DBL AND PLECKSTRIN HOMOLOGY DOMAINS FROM HSOS1

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS
Wavelength(s)	0.9686, 0.9792, 0.9794, 0.9856
Spacegroup name	C 1 2 1
Unit cell lengths	104.400, 70.440, 73.830
Unit cell angles	90.00, 96.29, 90.00

Refinement procedure

Resolution	30.000 - 2.300
R-factor	0.229
Rwork	0.229
R-free	0.26900
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.256
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS (CNS-03)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.036 *	0.187 *
Total number of observations	238642 *
Number of reflections	23805
<I/σ(I)>	21.1	5
Completeness [%]	99.0	96.9
Redundancy	19

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.5	20 *	5 MG/ML PROTEIN 100 MM BIS-TRIS PH 6.5 1-3% PEG6000 1 MM DTT

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	PEG6000	1-3 (%)
3	1	drop	Bis-Tris	100 (mM)	pH6.5
4	1	reservoir	PEG6000	1-3 (%)
5	1	reservoir	Bis-Tris	100 (mM)