1DBG
CRYSTAL STRUCTURE OF CHONDROITINASE B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-11-11 |
Detector | RIGAKU RAXIS II |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.600, 74.400, 58.700 |
Unit cell angles | 90.00, 92.90, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.700 |
R-factor | 0.19 |
Rwork | 0.180 |
R-free | 0.21000 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.000 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.047 | 0.142 |
Total number of observations | 241256 * | |
Number of reflections | 44806 * | |
<I/σ(I)> | 20 | |
Completeness [%] | 93.7 | 58.5 |
Redundancy | 2.9 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.7 | 292 * | Li, Y., (1999) Acta Crystallog. Sect., D55, 1055. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6-8 (mg/ml) | |
2 | 1 | reservoir | PEG8000 | 19 (%(w/v)) | |
3 | 1 | reservoir | Bicine | 100 (mM) | |
4 | 1 | reservoir | Tris-HCl | 100 (mM) | |
5 | 1 | reservoir | ammonium acetate | 0.15 (M) | |
6 | 1 | reservoir | MPD | 15 (%(v/v)) |