1DB2
CRYSTAL STRUCTURE OF NATIVE PLASMINOGEN ACTIVATOR INHIBITOR-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-12-20 |
Detector | MARRESEARCH |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 92.600, 92.600, 251.500 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.700 |
R-factor | 0.255 * |
Rwork | 0.255 |
R-free | 0.29900 |
RMSD bond length | 0.010 * |
RMSD bond angle | 1.700 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 * | 2.590 |
High resolution limit [Å] | 2.700 * | 2.700 * |
Rmerge | 0.078 * | 0.383 * |
Total number of observations | 603807 * | |
Number of reflections | 35572 * | |
<I/σ(I)> | 5.5 | |
Completeness [%] | 97.8 * | 90.1 * |
Redundancy | 16.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.6 | 4 * | used to seeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | 200 (mM) | ||
2 | 1 | reservoir | sodium potassium phosphate | 10 (mM) | |
3 | 1 | drop | protein |