1DB2

CRYSTAL STRUCTURE OF NATIVE PLASMINOGEN ACTIVATOR INHIBITOR-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-12-20
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
Unit cell lengths	92.600, 92.600, 251.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.700
R-factor	0.255 *
Rwork	0.255
R-free	0.29900
RMSD bond length	0.010 *
RMSD bond angle	1.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000 *	2.590
High resolution limit [Å]	2.700 *	2.700 *
Rmerge	0.078 *	0.383 *
Total number of observations	603807 *
Number of reflections	35572 *
<I/σ(I)>	5.5
Completeness [%]	97.8 *	90.1 *
Redundancy	16.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.6	4 *	used to seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		200 (mM)
2	1	reservoir	sodium potassium phosphate	10 (mM)
3	1	drop	protein