1DB1
CRYSTAL STRUCTURE OF THE NUCLEAR RECEPTOR FOR VITAMIN D COMPLEXED TO VITAMIN D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-07-11 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.193, 52.443, 133.286 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| Rwork | 0.191 |
| R-free | 0.21400 |
| RMSD bond length | 0.004 * |
| RMSD bond angle | 1.083 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.061 | 0.240 |
| Number of reflections | 29434 | |
| <I/σ(I)> | 22.1 | |
| Completeness [%] | 97.4 | 95.3 |
| Redundancy | 4.1 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 4 * | ammonium sulfate, mes, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4-10 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 0.7 (M) | |
| 3 | 1 | drop | MES | 50 (mM) | |
| 4 | 1 | drop | Tris-HCl | 5 (mM) | |
| 5 | 1 | drop | dithiothreitol | 5 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 1.4 (M) | |
| 7 | 1 | reservoir | MES | 0.1 (M) |
