1DAW
CRYSTAL STRUCTURE OF A BINARY COMPLEX OF PROTEIN KINASE CK2 (ALPHA-SUBUNIT) AND MG-AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-10-20 |
| Detector | MAC Science DIP-2030 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 143.110, 59.200, 45.810 |
| Unit cell angles | 90.00, 103.56, 90.00 |
Refinement procedure
| Resolution | 69.000 - 2.200 |
| R-factor | 0.212 * |
| Rwork | 0.212 |
| R-free | 0.28500 |
| Starting model (for MR) | 1A6O |
| RMSD bond length | 0.011 * |
| RMSD bond angle | 2.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.070 | 0.312 |
| Number of reflections | 19021 | |
| <I/σ(I)> | 18.6 | |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 * | 292 * | Guerra, B., (1998) Acta Crystallogr., Sect.D, 54, 143. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | drop | 500 (mM) | ||
| 3 | 1 | drop | Tris-HCl | 25 (mM) | |
| 4 | 1 | drop | 2-mercaptoethanol | 7 (mM) | |
| 5 | 1 | reservoir | ATP | 6 (mM) | |
| 6 | 1 | reservoir | 1.5 (mM) |
