1DAT

CUBIC CRYSTAL STRUCTURE RECOMBINANT HORSE L APOFERRITIN

Experimental procedure

Source type	SYNCHROTRON
Source details	LURE BEAMLINE D41A
Synchrotron site	LURE
Beamline	D41A
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1995-06-02
Detector	MARRESEARCH
Spacegroup name	F 4 3 2
Unit cell lengths	183.700, 183.700, 183.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.050
R-factor	0.179 *
Rwork	0.178
Starting model (for MR)	1ier
RMSD bond length	0.013
RMSD bond angle	2.820 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((AGROVATA)
Phasing software	X-PLOR (2.1)
Refinement software	X-PLOR (2.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.050	2.170
High resolution limit [Å]	2.050	2.050
Rmerge	0.096 *	0.278 *
Total number of observations	179720 *
Number of reflections	16931 *
<I/σ(I)>	6.4	2.7
Completeness [%]	99.6	99.6
Redundancy	10.6	9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop		3 (mM)
3	1	reservoir	ammonium sulfate	0.5 (M)
4	1	reservoir	cadmium sulfate	0.04 (M)