1DAP
C. GLUTAMICUM DAP DEHYDROGENASE IN COMPLEX WITH NADP+
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 290 |
| Detector technology | AREA DETECTOR |
| Collection date | 1995-09-20 |
| Detector | SIEMENS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.900, 65.800, 84.500 |
| Unit cell angles | 90.00, 106.60, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| Rwork | 0.170 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.765 |
| Data reduction software | XENGEN |
| Data scaling software | XENGEN |
| Phasing software | PHASES |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.078 * | 0.221 * |
| Total number of observations | 135992 * | |
| Number of reflections | 36359 | 4374 * |
| <I/σ(I)> | 7.7 | 2 |
| Completeness [%] | 89.2 | 64.8 |
| Redundancy | 3.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | Reddy, S.G., (1996) Proteins: Struct.,Funct., Genet., 25, 514. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DapDH | 20 (mg/ml) | |
| 2 | 1 | drop | HEPES | 20 (mM) | |
| 3 | 1 | drop | NADP+ | 1.5-2.0 (M) | |
| 4 | 1 | drop | PEG8000 | 13-17 (%) | |
| 5 | 1 | drop | sodium cacodylate | 100 (mM) | |
| 6 | 1 | drop | 150-300 (mM) | ||
| 7 | 1 | reservoir | PEG8000 | 13-17 (%) | |
| 8 | 1 | reservoir | sodium cacodylate | 100 (mM) | |
| 9 | 1 | reservoir | 150-300 (mM) |
