1DAD

DETHIOBIOTIN SYNTHETASE COMPLEXED WITH ADP

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1994-05-03
Detector	RIGAKU
Spacegroup name	C 1 2 1
Unit cell lengths	73.200, 49.200, 61.800
Unit cell angles	90.00, 107.10, 90.00

Refinement procedure

Resolution	6.000 - 1.600
R-factor	0.183
Rwork	0.183
RMSD bond length	0.014
RMSD bond angle	24.500 *
Data reduction software	R-AXIS
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.600 *
Rmerge	0.051
Total number of observations	91245 *
Number of reflections	25367
Completeness [%]	87.0
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	20 (mg/ml)
2	1	reservoir		0.2 (M)
3	1	reservoir	PEG8000	13-18 (%)
4	1	reservoir	cacodylate	0.1 (M)