1D9Q
OXIDIZED PEA FRUCTOSE-1,6-BISPHOSPHATASE FORM 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.940, 113.990, 94.810 |
| Unit cell angles | 90.00, 114.38, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.400 |
| Rwork | 0.186 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1spi |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.360 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.420 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.045 | |
| Total number of observations | 242800 * | |
| Number of reflections | 52402 | |
| <I/σ(I)> | 15.7 | |
| Completeness [%] | 89.0 | 84 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 290 | 25% PEG 400, 50MM NA ACETATE (PH 5.5), 50MM MGCL2, 5MM F6P, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG400 | 25 (%) | |
| 2 | 1 | reservoir | sodium acetate | 50 (mM) | |
| 3 | 1 | reservoir | 50 (mM) | ||
| 4 | 1 | reservoir | F6P | 5 (mM) |
