1D9H
Structural origins of the exonuclease resistance of a zwitterionic RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.170, 42.770, 45.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.600 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.24300 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.070 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.600 |
| Rmerge | 0.086 |
| Number of reflections | 6444 |
| Completeness [%] | 94.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 | 295 | MPD, POTASSIUN CACODYLATE, KCL, SPERMINE, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | POTASSIUN CACODYLATE | ||
| 2 | 1 | 1 | KCL | ||
| 3 | 1 | 1 | SPERMINE | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2 (mM) | |
| 2 | 1 | drop | potassium cacodylate | 40 (mM) | |
| 3 | 1 | drop | 80 (mM) | ||
| 4 | 1 | drop | spermine | 12 (mM) | |
| 5 | 1 | drop | 10 (%(v/v)) | ||
| 6 | 1 | reservoir | MPD | 35 (%) |
