1D9G
BOVINE INTERFERON-GAMMA AT 2.9 ANGSTROMS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-01-28 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.600, 79.600, 82.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.900 |
| R-factor | 0.229 |
| Rwork | 0.218 |
| R-free | 0.30800 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.330 |
| Data reduction software | XDS |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.062 | 0.260 |
| Number of reflections | 6450 | |
| <I/σ(I)> | 26.3 | |
| Completeness [%] | 97.1 | 87.7 |
| Redundancy | 3.75 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | MES, sodium chloride, ammonium sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 40 (%sat) | |
| 2 | 1 | drop | protein | 4 (mg/ml) | |
| 3 | 1 | drop | MES | 50 (mM) | |
| 4 | 1 | drop | 0.5 (M) |
