1D9F

CRYSTAL STRUCTURE OF THE COMPLEX OF DNA POLYMERASE I KLENOW FRAGMENT WITH DNA TETRAMER CARRYING 2'-O-(3-AMINOPROPYL)-RNA MODIFICATION 5'-D(TT)-AP(U)-D(T)-3'

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 5ID-B
Synchrotron site	APS
Beamline	5ID-B
Temperature [K]	120
Detector technology	CCD
Collection date	1998-10-30
Detector	MARRESEARCH
Spacegroup name	P 43
Unit cell lengths	102.650, 102.650, 86.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.000
R-factor	0.224
Rwork	0.224
R-free	0.24300
RMSD bond length	0.009
RMSD bond angle	1.630
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	35.000
High resolution limit [Å]	3.000 *
Rmerge	0.078
Number of reflections	20110
Completeness [%]	99.9
Redundancy	4.23

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7 *	295	SODIUM CITRATE, pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 295K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	SODIUM CITRATE

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2 (mM)
2	1	drop	potassium cacodylate	40 (mM)
3	1	drop		80 (mM)
4	1	drop	spermine	12 (mM)
5	1	drop	MPD	10 (%(v/v))
6	1	reservoir	MPD	35 (%)