1D9C

BOVINE INTERFERON-GAMMA AT 2.0 ANGSTROMS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	36.150, 81.080, 83.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.000
R-factor	0.201
Rwork	0.201
R-free	0.27500
RMSD bond length	0.013
RMSD bond angle	21.400 *
Data reduction software	XDS
Phasing software	PHASES
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.000	2.070
High resolution limit [Å]	2.000	2.020
Rmerge	0.030
Total number of observations	71855 *
Number of reflections	16274 *
<I/σ(I)>	72.5
Completeness [%]	97.0	86.5
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	MES, sodium chloride, sodium citrate , pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium citrate	50 (%sat)