1D97
CHIRAL PHOSPHOROTHIOATE ANALOGUES OF B-DNA: THE CRYSTAL STRUCTURE OF RP-D(GP(S) CPGP(S)CPGP(S)C)
Experimental procedure
| Temperature [K] | 291 |
| Detector technology | DIFFRACTOMETER |
| Detector | SYNTEX P21 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.900, 39.150, 20.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.170 |
| R-factor | 0.145 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 2.170 |
| Rmerge | 0.240 * |
| Number of reflections | 1954 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 14 * | VAPOR DIFFUSION, temperature 287.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | ISOPROPANOL | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 1 | SPERMINE_HCL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | oligonucleotide | 1 (mM) | can be replaced with ammonium salt |
| 2 | 1 | drop | sodium cacodylate | 100 (mM) | |
| 3 | 1 | drop | 0.75 (mM) | ||
| 4 | 1 | drop | spermine hydrochloride | 1.25 (mM) | |
| 5 | 1 | reservoir | isopropanol | 10 (%(v/v)) |
