1D93
STRUCTURAL VARIATION IN D(CTCTAGAG). IMPLICATIONS FOR PROTEIN-DNA INTERACTIONS
Experimental procedure
| Detector technology | DIFFRACTOMETER |
| Detector | SYNTEX P21 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 42.520, 42.520, 24.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.150 |
| R-factor | 0.147 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.150 |
| Rmerge | 0.075 * |
| Number of reflections | 1378 |
| Completeness [%] | 70.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 18 * | pH 6.80, VAPOR DIFFUSION, SITTING DROP, temperature 291.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 1 | SPERMINE_HCL | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | octamer | 6 (mg/ml) | |
| 2 | 1 | drop | sodium cacodylate | 60 (mM) | |
| 3 | 1 | drop | magnesium chloride | 25 (mM) | |
| 4 | 1 | drop | spermine hydrochloride | 1 (mM) | |
| 5 | 1 | drop | MPD | 7 (%(v/v)) | |
| 6 | 1 | reservoir | MPD | 50 (%(v/v)) |
