1D8U
CRYSTAL STRUCTURE OF NON-SYMBIOTIC PLANT HEMOGLOBIN FROM RICE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-08-21 |
| Detector | FUJI |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 126.800, 126.800, 55.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.350 |
| R-factor | 0.208 * |
| Rwork | 0.208 |
| R-free | 0.26100 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 2.110 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.420 |
| High resolution limit [Å] | 2.200 | 2.300 * |
| Rmerge | 0.690 * | 0.223 * |
| Number of reflections | 21422 * | 1634 * |
| <I/σ(I)> | 13.7 | |
| Completeness [%] | 80.0 * | 46 * |
| Redundancy | 8.06 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 295 | AMMONIUM PHOSPHATE, SUCROSE, POTASSIUM PHOSPHATE, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 22K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium phosphate | 2.2 (M) | |
| 2 | 1 | reservoir | sucrose | 20 (%) | |
| 3 | 1 | drop | potassium phosphate | 10 (mM) | |
| 4 | 1 | drop | protein | 2.2 (mM) |
