1D8S
ESCHERICHIA COLI F1 ATPASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 180.680, 197.520, 237.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 4.400 |
| RMSD bond length | 3.020 |
| RMSD bond angle | 0.107 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 4.440 |
| High resolution limit [Å] | 4.400 | 4.400 |
| Rmerge | 0.122 | 0.358 |
| Total number of observations | 89801 * | |
| Number of reflections | 17553 | |
| <I/σ(I)> | 3.7 | |
| Completeness [%] | 64.5 | 53.6 |
| Redundancy | 5.1 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 25 * | Tris, PEG 8000, glycerol, NaCl, MgSO4, LiSO4, NaN3, EDTA, AMP-PNP, ATP, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 10 | 1 | drop | ATP | 0.1 (M) | |
| 11 | 1 | drop | PEG8000 | 8.0 (%) | |
| 12 | 1 | reservoir | ammonium sulfate | 35 (%sat) | |
| 2 | 1 | drop | Tris-HCl | 110 (mM) | |
| 3 | 1 | drop | glycerol | 10 (%) | |
| 4 | 1 | drop | 100 (mM) | ||
| 5 | 1 | drop | 10 (mM) | ||
| 6 | 1 | drop | 40 (mM) | ||
| 7 | 1 | drop | 0.05 (%) | ||
| 8 | 1 | drop | EDTA | 0.1 (M) | |
| 9 | 1 | drop | adenylylimidodiphosphate | 5 (mM) |
