1D8L

E. COLI HOLLIDAY JUNCTION BINDING PROTEIN RUVA NH2 REGION LACKING DOMAIN III

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	OTHER
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1998-01-22
Detector	MAC Science DIP-2020
Spacegroup name	P 21 21 2
Unit cell lengths	79.600, 115.600, 34.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.500
R-factor	0.243 *
Rwork	0.243
R-free	0.32000
RMSD bond length	0.006 *
RMSD bond angle	1.500 *
Data reduction software	DENZO
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	2.500
Rmerge	0.081	0.309 *
Total number of observations	49960 *
Number of reflections	9523 *
<I/σ(I)>	17.3
Completeness [%]	85.6	79.3 *
Redundancy	5.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	4.6	20 *	pH 4.60

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	citrate-NaOH	50 (mM)
2	1	1	dithiothreitol	1 (mM)
3	1	1	glycerol	10 (%)