1D8C
MALATE SYNTHASE G COMPLEXED WITH MAGNESIUM AND GLYOXYLATE
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-07-18 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.800, 88.700, 109.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-free | 0.28000 * |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 15.960 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.052 | 0.307 |
| Total number of observations | 367712 * | |
| Number of reflections | 93570 | |
| <I/σ(I)> | 24.7 | 2.7 |
| Completeness [%] | 99.4 | 98 |
| Redundancy | 3.93 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | used to seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 1.8 (M) | |
| 2 | 1 | reservoir | imidazole | 100 (mM) | pH8.0 |
| 3 | 1 | reservoir | sorbitol | 50 (mM) | |
| 4 | 1 | drop | enzyme | 24 (mg/ml) | |
| 5 | 1 | drop | 14 (mM) | ||
| 6 | 1 | drop | dithiothreitol | 7 (mM) |
