1D87
CONFORMATIONAL INFLUENCE OF THE RIBOSE 2'-HYDROXYL GROUP: CRYSTAL STRUCTURES OF DNA-RNA CHIMERIC DUPLEXES
Experimental procedure
Source type | ROTATING ANODE |
Temperature [K] | 273 |
Detector technology | DIFFRACTOMETER |
Detector | RIGAKU AFC-5R |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 27.500, 44.230, 44.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | ? - 2.250 |
R-factor | 0.178 |
RMSD bond length | 0.020 |
Refinement software | PROLSQ ((MODIFIED BY G.J.QUIGLEY)) |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.250 |
Number of reflections | 1592 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 18 * | Egli, M., (1992) Proc.Nat.Acad.Sci.USA, 89, 534. * |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
2 | 1 | 1 | NA CACODYLATE | ||
3 | 1 | 1 | MGCL2 | ||
4 | 1 | 1 | SPERMINE | ||
5 | 1 | 2 | WATER | ||
6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | oligonucleotide | 1.5 (mM) | single strand concentration |
2 | 1 | drop | sodium cacodylate | 30 (mM) | |
3 | 1 | drop | magnesium chloride | 7.5 (mM) | |
4 | 1 | drop | spermine tetrachloride | 7.5 (mM) | |
5 | 1 | reservoir | MPD | 40 (%(v/v)) |