1D82
CRYSTAL AND MOLECULAR STRUCTURE OF D(GTCTAGAC)
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 295 |
| Detector technology | DIFFRACTOMETER |
| Detector | RIGAKU AFC-5 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 42.560, 42.560, 24.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.500 |
| R-factor | 0.211 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 1757 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.8 | 295 | pH 6.80, VAPOR DIFFUSION, temperature 295.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 1 | SPERMINE_HCL | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 6 (mg/ml) | |
| 2 | 1 | drop | sodium cacodylate | 60 (mM) | |
| 3 | 1 | drop | magnesium cacodylate | 25 (mM) | |
| 4 | 1 | drop | spermine tetrachloride | 6 (mM) | |
| 5 | 1 | drop | MPD | 10 (%(v/v)) | |
| 6 | 1 | reservoir | MPD | 50 (%) |
