1D7P
Crystal structure of the c2 domain of human factor viii at 1.5 a resolution at 1.5 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 150 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-11-22 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.440, 56.410, 65.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.500 |
| R-factor | 0.206 |
| Rwork | 0.205 |
| R-free | 0.21500 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.470 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.040 | 0.188 |
| Number of reflections | 27327 | |
| <I/σ(I)> | 13.7 | |
| Completeness [%] | 96.0 | 93.9 |
| Redundancy | 4.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6 * | 298 | AMMONIUM SULFATE, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
| 1 | unknown * | 6 * | 298 | AMMONIUM SULFATE, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
| 1 | unknown * | 6 * | 298 | AMMONIUM SULFATE, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
| 1 | unknown * | 6 * | 298 | AMMONIUM SULFATE, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | ammonium sulfate | 2 (M) | |
| 2 | 1 | 1 | MES | 0.1 (M) | |
| 3 | 1 | 1 | 0.015 (%) |
