1D7M
COILED-COIL DIMERIZATION DOMAIN FROM CORTEXILLIN I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-11-14 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 70.420, 128.650, 91.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.700 |
| R-factor | 0.218 * |
| Rwork | 0.205 |
| R-free | 0.25700 * |
| RMSD bond length | 0.170 * |
| RMSD bond angle | 1.770 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.078 * | 0.462 |
| Total number of observations | 49645 * | |
| Number of reflections | 11302 | |
| <I/σ(I)> | 5.3 | |
| Completeness [%] | 94.6 * | 88.2 |
| Redundancy | 2.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 297 | Burkhard, P., (1998) J.Struct.Biol., 122, 293. * |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 297 | Burkhard, P., (1998) J.Struct.Biol., 122, 293. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium phosphate | 5 (mM) | |
| 2 | 1 | drop | protein | 15 (mg/ml) | |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | reservoir | ammonium sulfate | 1.5 (M) | |
| 5 | 1 | reservoir | Tris | 0.1 (M) | |
| 6 | 1 | reservoir | PEG400 | 2.5 (%) | |
| 7 | 1 | reservoir | dioxane | 1 (%) |
