1D7K

CRYSTAL STRUCTURE OF HUMAN ORNITHINE DECARBOXYLASE AT 2.1 ANGSTROMS RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-01-15
Detector	FUJI
Spacegroup name	P 21 21 21
Unit cell lengths	61.680, 107.450, 139.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.500 - 2.100
Rwork	0.212
R-free	0.28800
RMSD bond length	0.016
RMSD bond angle	2.500
Data reduction software	DENZO
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.160
High resolution limit [Å]	2.100	2.090
Rmerge	0.068	0.363
Total number of observations	223151 *
Number of reflections	53656
<I/σ(I)>	10.1
Completeness [%]	96.4	97.5
Redundancy	4	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5	289	20% PEG 3350, 0.2M NaCl, 5mM DTT, 0.1M Tris-HCl, pH 7.5, VAPOR DIFFUSION, temperature 16K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	20 (mg/ml)
2	1	2	PEG3350	20 (%(w/v))
3	1	2	Tris-HCl	0.1 (M)
4	1	2		0.2 (M)