1D7D
CYTOCHROME DOMAIN OF CELLOBIOSE DEHYDROGENASE, HP3 FRAGMENT, PH 7.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-11-24 |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 |
| Unit cell lengths | 138.300, 138.300, 53.390 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 1.900 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.22100 |
| RMSD bond length | 0.001 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.084 | 0.329 |
| Total number of observations | 236651 * | |
| Number of reflections | 45909 | |
| <I/σ(I)> | 16.7 | |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 5.2 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | drop consists of equal amounts of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-10 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 30-40 (%) | |
| 3 | 1 | reservoir | MPD | 5-20 (%) | |
| 4 | 1 | reservoir | HEPES | 100 (mM) | |
| 5 | 1 | reservoir | 10-100 (mM) |
