1D7C
CYTOCHROME DOMAIN OF CELLOBIOSE DEHYDROGENASE, PH 4.6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-02-06 |
| Detector | ADSC |
| Spacegroup name | P 65 |
| Unit cell lengths | 139.400, 139.400, 53.940 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 1.900 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.22000 |
| RMSD bond length | 0.001 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.047 | 0.151 |
| Total number of observations | 228007 * | |
| Number of reflections | 46194 | |
| <I/σ(I)> | 28.1 | |
| Completeness [%] | 97.5 | 93.7 |
| Redundancy | 4.9 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | drop consists of equal amounts of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-10 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 30-40 (%) | |
| 3 | 1 | reservoir | MPD | 5-20 (%) | |
| 4 | 1 | reservoir | 100 (mM) | ||
| 5 | 1 | reservoir | 10-100 (mM) |
