1D79
HIGH RESOLUTION REFINEMENT OF THE HEXAGONAL A-DNA OCTAMER D(GTGTACAC) AT 1.4 ANGSTROMS RESOLUTION
Experimental procedure
| Temperature [K] | 258 |
| Detector technology | AREA DETECTOR |
| Detector | XENTRONICS |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 32.341, 32.341, 78.490 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 * - 1.450 |
| R-factor | 0.191 |
| RMSD bond length | 0.043 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 5.000 |
| High resolution limit [Å] | 1.450 |
| Rmerge | 0.052 * |
| Total number of observations | 9036 * |
| Number of reflections | 3292 |
| Completeness [%] | 67.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 283 | VAPOR DIFFUSION, temperature 283.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | SPERMIDINE | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD | ||
| 7 | 1 | 2 | MGCL2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | MPD | 20 (%(v/v)) | |
| 2 | 1 | drop | 4 (mM) | ||
| 3 | 1 | drop | spermine | 2 (mM) | |
| 4 | 1 | drop | DNA | duplex | |
| 5 | 1 | reservoir | MPD | 50 (%) | |
| 6 | 1 | reservoir | 20 (mM) |
