1D79
HIGH RESOLUTION REFINEMENT OF THE HEXAGONAL A-DNA OCTAMER D(GTGTACAC) AT 1.4 ANGSTROMS RESOLUTION
Experimental procedure
Temperature [K] | 258 |
Detector technology | AREA DETECTOR |
Detector | XENTRONICS |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 32.341, 32.341, 78.490 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 * - 1.450 |
R-factor | 0.191 |
RMSD bond length | 0.043 |
Refinement software | NUCLSQ |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 5.000 |
High resolution limit [Å] | 1.450 |
Rmerge | 0.052 * |
Total number of observations | 9036 * |
Number of reflections | 3292 |
Completeness [%] | 67.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 283 | VAPOR DIFFUSION, temperature 283.00K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
2 | 1 | 1 | MPD | ||
3 | 1 | 1 | MGCL2 | ||
4 | 1 | 1 | SPERMIDINE | ||
5 | 1 | 2 | WATER | ||
6 | 1 | 2 | MPD | ||
7 | 1 | 2 | MGCL2 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | MPD | 20 (%(v/v)) | |
2 | 1 | drop | 4 (mM) | ||
3 | 1 | drop | spermine | 2 (mM) | |
4 | 1 | drop | DNA | duplex | |
5 | 1 | reservoir | MPD | 50 (%) | |
6 | 1 | reservoir | 20 (mM) |