1D75
CONFORMATION OF THE GUANINE.8-OXOADENINE BASE PAIRS IN THE CRYSTAL STRUCTURE OF D(CGCGAATT(O8A)GCG)
Experimental procedure
| Source type | ROTATING ANODE |
| Temperature [K] | 297 |
| Detector technology | DIFFRACTOMETER |
| Detector | RIGAKU AFC-5 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.480, 41.840, 64.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.000 - 2.800 |
| R-factor | 0.151 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.016 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.240 * |
| Number of reflections | 3485 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 277 | pH 7.40, VAPOR DIFFUSION, SITTING DROP, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | MG ACETATE | ||
| 5 | 1 | 1 | SPERMINE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 1 (mM) | |
| 2 | 1 | drop | sodium cacodylate | 20 (mM) | |
| 3 | 1 | reservoir | magnesium acetate | 12 (mM) | |
| 4 | 1 | reservoir | spermine tetrahydrochloride | 12 (mM) | |
| 5 | 1 | reservoir | MPD | 9 (%(v/v)) |
