1D6Q

HUMAN LYSOZYME E102 MUTANT LABELLED WITH 2',3'-EPOXYPROPYL GLYCOSIDE OF N-ACETYLLACTOSAMINE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR571
Temperature [K]	288
Detector technology	DIFFRACTOMETER
Collection date	1999-05-15
Detector	ENRAF-NONIUS FAST
Spacegroup name	P 21 21 21
Unit cell lengths	56.790, 61.240, 33.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.960
R-factor	0.184
Rwork	0.184
R-free	0.24800
RMSD bond length	0.011
RMSD bond angle	24.900 *
Data reduction software	MERGEF ((BY K. HARATA))
Data scaling software	MERGEF ((K.HARATA))
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.450	2.020
High resolution limit [Å]	1.960	1.960
Rmerge	0.149	0.276
Number of reflections	41028
Completeness [%]		94 *
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	298	5M NH4NO3 IN 10 MM NAOAC (PH4.5), VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	sodium acetate	25 (mM)
3	1	drop	ammonium nitrate	3 (M)
4	1	reservoir	sodium acetate	25 (mM)
5	1	reservoir	ammonium nitrate	5 (M)