1D6P

HUMAN LYSOZYME L63 MUTANT LABELLED WITH 2',3'-EPOXYPROPYL N,N'-DIACETYLCHITOBIOSE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR571
Temperature [K]	288
Detector technology	DIFFRACTOMETER
Collection date	1999-05-10
Detector	ENRAF-NONIUS FAST
Spacegroup name	P 21 21 21
Unit cell lengths	58.200, 60.780, 32.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.230
R-factor	0.181
Rwork	0.181
R-free	0.21600
RMSD bond length	0.011
RMSD bond angle	25.300 *
Data reduction software	MERGEF ((BY K.HARATA))
Data scaling software	MERGEF ((K.HARATA))
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.270	2.320
High resolution limit [Å]	2.230	2.230
Rmerge	0.100	0.293
Number of reflections	40267
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	298	5M NH4NO3 IN 10 MM NAOAC(PH 4.5), VAPOR DIFFUSION, SITTING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	sodium acetate	25 (mM)
3	1	drop	ammonium nitrate	3 (M)
4	1	reservoir	sodium acetate	25 (mM)
5	1	reservoir	ammonium nitrate	5 (M)