1D66
DNA RECOGNITION BY GAL4: STRUCTURE OF A PROTEIN/DNA COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 118 |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 80.850, 80.850, 73.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.700 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.900 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.700 |
| Rmerge | 0.075 * |
| Completeness [%] | 94.0 * |
| Redundancy | 4.2 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.8 | pH 6.80, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | CACL2 | ||
| 4 | 1 | 1 | NACL | ||
| 5 | 1 | 1 | NA CACODYLATE | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.6 (mM) | dimer |
| 10 | 1 | reservoir | sodium cacodylate | 20 (mM) | |
| 2 | 1 | drop | DNA | 0.9 (mM) | duplex |
| 3 | 1 | drop | MPD | 16 (%) | |
| 4 | 1 | drop | 75 (mM) | ||
| 5 | 1 | drop | 24 (mM) | ||
| 6 | 1 | drop | sodium cacoylate | 12 (mM) | |
| 7 | 1 | reservoir | 27 (%) | ||
| 8 | 1 | reservoir | 125 (mM) | ||
| 9 | 1 | reservoir | 40 (mM) |
