1D5Y
CRYSTAL STRUCTURE OF THE E. COLI ROB TRANSCRIPTION FACTOR IN COMPLEX WITH DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-01-01 |
| Detector | OTHER |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.384, 208.012, 67.731 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.700 |
| R-factor | 0.254 |
| Rwork | 0.254 |
| R-free | 0.30200 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.360 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.082 | 0.426 |
| Total number of observations | 306889 * | |
| Number of reflections | 54476 | |
| <I/σ(I)> | 8.7 | |
| Completeness [%] | 95.1 | 87.2 |
| Redundancy | 6 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.5 | 295 | Peg 8000, MES, MgCl2, glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MES | ||
| 2 | 1 | 1 | MGCL2 | ||
| 3 | 1 | 1 | GLYCEROL | ||
| 4 | 1 | 1 | PEG 8000 | ||
| 5 | 1 | 2 | GLYCEROL | ||
| 6 | 1 | 2 | PEG 8000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | reservoir | MES | 100 (mM) | |
| 3 | 1 | reservoir | 125 (mM) | ||
| 4 | 1 | reservoir | glycerol | 10 (%(v/v)) | |
| 5 | 1 | reservoir | dithiothreitol | 1 (mM) | |
| 6 | 1 | reservoir | spermine-HCl | 1 (mM) | |
| 7 | 1 | reservoir | PEG8000 | 15 (%(w/v)) |
