1D5I
UNLIGANDED GERMLINE PRECURSOR OF AN OXY-COPE CATALYTIC ANTIBODY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 1998-02-16 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.170, 64.130, 75.950 |
| Unit cell angles | 90.00, 99.53, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.26400 * |
| RMSD bond length | 0.019 * |
| RMSD bond angle | 2.070 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.040 * | 0.140 |
| Total number of observations | 74105 * | |
| Number of reflections | 30863 | |
| <I/σ(I)> | 23 | |
| Completeness [%] | 90.3 | 90.5 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 75mM Hepes pH 7.0 9% PEG 4000 150 mM Ammonium Sulfate 20% 1,4 butanediol 100 mM Cadmium Chloride, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | cadmium chloride | 80 (mM) | |
| 2 | 1 | drop | TSA/I | 2 (mM) | |
| 3 | 1 | drop | HEPES | 75 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 250 (mM) | |
| 5 | 1 | reservoir | 1,4-butanediol | 20 (%) | |
| 6 | 1 | reservoir | PEG4000 | 9 (%) |
