1D5H

Rnase s(f8a). mutant ribonucleasE S.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1994-03-08
Detector	XENTRONICS
Spacegroup name	P 31 2 1
Unit cell lengths	44.710, 44.710, 97.520
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.250
R-factor	0.21
Rwork	0.210
R-free	0.25700
RMSD bond length	0.028
RMSD bond angle	2.800
Data reduction software	X-GEN
Data scaling software	X-GEN
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.370
High resolution limit [Å]	2.200	2.250
Rmerge	0.060	0.300
Number of reflections	5328
<I/σ(I)>	13
Completeness [%]	95.0	70
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.75	293	Thomson, J., (1994) Biochemistry, 33, 8587. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4-5 (mg/ml)
2	1	drop		3 (M)
3	1	drop	acetate	0.1 (M)
4	1	drop	ammonium sulfate	35 (%)