1D57

ALTERNATIVE STRUCTURES FOR ALTERNATING POLY(DA-DT) TRACTS: THE STRUCTURE OF THE B-DNA DECAMER C-G-A-T-A-T-A-T-C-G

Experimental procedure

Source type	ROTATING ANODE
Temperature [K]	273
Detector technology	DIFFRACTOMETER
Detector	RIGAKU AFC-5R
Spacegroup name	P 21 21 21
Unit cell lengths	38.690, 39.560, 33.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.165
Refinement software	NUCLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000
High resolution limit [Å]	2.000	2.000 *
Number of reflections	1852
Completeness [%]		17 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.9	4 *	The reservoir was bugun at 15% MPD and increased 3% per week. *

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	WATER
2	1	1	MPD
3	1	1	MG ACETATE
4	1	1	SPERMINE_HCL
5	1	2	WATER
6	1	2	MPD

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	DNA	0.33 (mM)
2	1	drop	magnesium acetate	15 (mM)
3	1	drop	spermine-HCl	0.34 (mM)
4	1	drop	MPD	10 (%(v/v))
5	1	reservoir	MPD	15 (%)