1D4X
Crystal Structure of Caenorhabditis Elegans Mg-ATP Actin Complexed with Human Gelsolin Segment 1 at 1.75 A resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-01-01 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 177.820, 68.989, 56.593 |
| Unit cell angles | 90.00, 104.15, 90.00 |
Refinement procedure
| Resolution | 25.000 * - 1.750 |
| R-factor | 0.2307 |
| Rwork | 0.199 |
| R-free | 0.23300 |
| RMSD bond length | 0.011 * |
| RMSD bond angle | 1.434 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.041 | 0.102 |
| Number of reflections | 64713 | |
| <I/σ(I)> | 24.2 | |
| Completeness [%] | 96.9 | 88.3 |
| Redundancy | 3.91 | 2.57 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 100 MM TRIS-HCL, PH 8.0, 2 MM MG-ATP, 1.60 M LI2SO4,, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | gelsolin | 1:1.25 stoichiometry | |
| 10 | 1 | reservoir | HEPES | 0.1 (M) | |
| 2 | 1 | drop | Tris-HCl | 2 (mM) | |
| 3 | 1 | drop | ATP | 2 (mM) | |
| 4 | 1 | drop | 4 (mM) | ||
| 5 | 1 | drop | EGTA | 0.5 (mM) | |
| 6 | 1 | drop | 1 (mM) | ||
| 7 | 1 | reservoir | 1.55 (M) | ||
| 8 | 1 | reservoir | Mg2+-ATP | 2 (mM) | |
| 9 | 1 | reservoir | EGTA | 0.5 (mM) |
