1D4T

CRYSTAL STRUCTURE OF THE XLP PROTEIN SAP IN COMPLEX WITH A SLAM PEPTIDE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Detector	PRINCETON 2K
Spacegroup name	P 1 21 1
Unit cell lengths	25.870, 42.910, 44.950
Unit cell angles	90.00, 98.26, 90.00

Refinement procedure

Resolution	8.000 - 1.100
R-factor	0.13
Rwork	0.115
R-free	0.16500
Structure solution method	AB INITIO
RMSD bond length	0.017 *
RMSD bond angle	0.032 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	SHELXL-97

Data quality characteristics

	Overall
Low resolution limit [Å]	22.000
High resolution limit [Å]	1.100 *
Rmerge	0.043
Total number of observations	134878 *
Number of reflections	34081 *
Completeness [%]	86.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	295	30% PEG 8000, 20% GLYCEROL, 100 MM HEPES PH 8.0, AND 100 MM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 22K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	HEPES	20 (mM)
3	1	drop		150 (mM)
4	1	drop	dithiothreitol	10 (mM)
5	1	reservoir	PEG8000	30 (%)
6	1	reservoir	glycerol	20 (%)
7	1	reservoir	HEPES	100 (mM)
8	1	reservoir	dithiothreitol	10 (mM)