1D4O
CRYSTAL STRUCTURE OF TRANSHYDROGENASE DOMAIN III AT 1.2 ANGSTROMS RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-03-21 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.660, 36.730, 38.490 |
| Unit cell angles | 68.36, 87.99, 74.80 |
Refinement procedure
| Resolution | 50.000 - 1.210 |
| R-factor | 0.167 |
| Rwork | 0.168 |
| R-free | 0.22300 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.700 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MLPHARE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.100 | 1.240 |
| High resolution limit [Å] | 1.210 | 1.210 |
| Rmerge | 0.068 | 0.301 |
| Total number of observations | 89452 * | |
| Number of reflections | 45717 | |
| <I/σ(I)> | 4 | |
| Completeness [%] | 91.2 | 72.3 |
| Redundancy | 2 | 91.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.8 * | 277 | 24% w/v MPEG 5000, 100mM sodium cacodylate and 200mM magnesium acetate., pH 7.4, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 50 (mM) | |
| 2 | 1 | drop | EDTA | 1 (mM) | |
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | drop | PMSF | 0.5 (mM) | |
| 5 | 1 | drop | protain | 10 (mg/ml) | |
| 6 | 1 | reservoir | mPEG5000 | 24 (%(w/v)) | |
| 7 | 1 | reservoir | sodium cacodylate | 100 (mM) | |
| 8 | 1 | reservoir | magnesium acetate | 200 (mM) |
