1D44
DNA DODECAMER C-G-C-G-A-A-T-T-C-G-C-G/HOECHST 33258 COMPLEX: 0 DEGREES C, PIPERAZINE DOWN
Experimental procedure
| Source type | ROTATING ANODE |
| Temperature [K] | 288 |
| Detector technology | DIFFRACTOMETER |
| Detector | RIGAKU AFC-5R |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 23.950, 38.990, 65.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.157 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 8.000 * |
| High resolution limit [Å] | 2.000 * |
| Rmerge | 0.178 * |
| Number of reflections | 2540 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.9 * | 4 * | pH 6.50, VAPOR DIFFUSION, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MG ACETATE | ||
| 4 | 1 | 1 | SPERMINE_HCL | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 0.22 (mM) | |
| 2 | 1 | drop | Hoechest 33258 | 0.44 (mM) | |
| 3 | 1 | drop | magnesium acetate | 5.0 (mM) | |
| 4 | 1 | drop | spermine-HCl | 0.5 (mM) | |
| 5 | 1 | drop | MPD | 15 (%(v/v)) | |
| 6 | 1 | reservoir | 32.5 (%(v/v)) |
