1D41
STABILIZATION OF Z-DNA BY DEMETHYLATION OF THYMINE BASES: 1.3 ANGSTROMS SINGLE-CRYSTAL STRUCTURE OF D(M5CGUAM5CG)
Experimental procedure
Detector technology | IMAGE PLATE |
Detector | RIGAKU |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 17.820, 30.440, 44.520 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | ? - 1.300 |
R-factor | 0.208 |
Starting model (for MR) | ZDFB06 IN WHICH THE METHYL GROUPS OF THE THYMINE BASES WERE REMOVED FROM THE C5 POSITIONS. |
Refinement software | NUCLSQ |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.300 |
Number of reflections | 2870 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | pH 7.00, VAPOR DIFFUSION |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
2 | 1 | 1 | MPD | ||
3 | 1 | 1 | NA CACODYLATE | ||
4 | 1 | 1 | MGCL2 | ||
5 | 1 | 2 | WATER | ||
6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | MPD | 30 (%) | |
2 | 1 | drop | oligonucleotide | 4.0 (mM) | |
3 | 1 | drop | sodium cacodylate | 28 (mM) | |
4 | 1 | drop | magnesium chloride | 15 (mM) | |
5 | 1 | drop | MPD | 8.5 (%) |