1D3W
Crystal structure of ferredoxin 1 d15e mutant from azotobacter vinelandii at 1.7 angstrom resolution.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-07-09 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 55.370, 55.370, 92.535 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.700 |
R-factor | 0.209 |
Rwork | 0.209 |
R-free | 0.24700 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.895 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.720 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.080 | 0.546 |
Number of reflections | 16030 | |
<I/σ(I)> | 17.38 | |
Completeness [%] | 96.5 | 89.5 |
Redundancy | 13.81 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 277 | Stout, C.D., (1989 J. Mol. Biol., 205, 545. * |