1D3V
CRYSTAL STRUCTURE OF THE BINUCLEAR MANGANESE METALLOENZYME ARGINASE COMPLEXED WITH 2(S)-AMINO-6-BORONOHEXANOIC ACID, AN L-ARGININE ANALOG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 1999-08-30 |
| Detector | OTHER |
| Spacegroup name | P 3 |
| Unit cell lengths | 91.300, 91.300, 69.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.157 |
| Rwork | 0.157 |
| R-free | 0.17900 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 23.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.053 | 0.125 |
| Total number of observations | 126870 * | |
| Number of reflections | 66822 * | |
| <I/σ(I)> | 22.9 | |
| Completeness [%] | 91.7 | 59.2 |
| Redundancy | 3.8 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 * | pH 8.10 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | arginase | 11 (mg/ml) | |
| 2 | 1 | drop | ABH | 2 (mM) | |
| 3 | 1 | drop | 5 (mM) | ||
| 4 | 1 | drop | beta-mercaptoethanol | 2 (mM) | |
| 5 | 1 | drop | Bicine | 25 (mM) | pH8.5 |
| 6 | 1 | reservoir | PEG1500 | 26 (%) | |
| 7 | 1 | reservoir | bicine | 100 (mM) | pH8.1 |
